Part:BBa_K5157059:Design
PETase-G4S-60-4-C
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 751
Illegal PstI site found at 952 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 751
Illegal PstI site found at 952 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 751
Illegal PstI site found at 952 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 751
Illegal PstI site found at 952
Illegal NgoMIV site found at 142 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed PET-binding peptide prediction with the help of deep learning, and, then, fused PETase with the efficient binding peptide obtained from the prediction and screening through linker to construct a fusion protein, so as to improve the binding ability of PETase to PET microplastics with the help of PET-binding peptide, thus improving the degradation efficiency.
Source
PETase is derived from the genome of Ideonella sakaiensis 201-F6, a bacterium capable of PET degradation and assimilation.
The PET-binding peptide was predicted by LSTM and GCN models from the database.
References
[1] Yoshida S, Hiraga K, Takehana T, et al. A bacterium that degrades and assimilates poly(ethylene terephthalate) [J]. Science, 2016, 351(6278): 1196-1199.
[2] Puspitasari N, Tsai S L, Lee C K. Fungal hydrophobin RolA enhanced PETase hydrolysis of polyethylene terephthalate [J]. Applied Biochemistry and Biotechnology, 2021, 193(5): 1284-1295.
[3] Chen X, Zaro J L, Shen W C. Fusion protein linkers: property, design and functionality [J]. Advanced Drug Delivery Reviews, 2013, 65(10): 1357-1369.